kasa 3 way switch
Chemical composition and antioxidant activity of Strobilanthes crispus leaf extract. SH treatment at the IC50 caused a 2.5-fold delay in cell proliferation while twice the IC50 of SH delayed cell doubling by 3.4-fold. *P<0.05, compared with the untreated control (representing 100% of cell viability). HCC is primarily caused by chronic viral hepatitis B or C infection, iron overload, aflotoxin exposure, obesity, alcohol-associated cirrhosis and possibly non-alcoholic fatty liver disease (3,4). Diuretic â One of many benefits of Strobilanthes Crispus is to increase the rate of urination, so it help to remove excess of water, salt and toxin. Ismail M, Manickam E, Danial AM, Rahmat A, Yahaya A. Microscopic observation of (A) untreated control and (B) treated HepG-2 liver cancer cells, and (C) untreated control and (D) treated MDA-MB-231 breast cancer cells. trans-Cinnamic acid at a concentration of 0.44 mg/g DM was detected in extracts of leaves from Penang but not in the extracts of the leaves from the other two locations. Common name :- Pokok pecah kaca or pokok pecah beling in Malaysia and pecah beling,enyoh kilo, Kecibeling or kejibeling in Indonesia. In the SH-treated HepG-2 cells, morphological changes, including the formation of vacuoles within the cell were observed. In order to identify the compounds responsible for the anti-cancer effects, further studies on the isolation and purification of the bioactive compounds from S. crispa are required. J Med Plants Res. Cells growing in the exponential phase were used for cell viability assay. 2012;41(4):403-409. 2002;79(3):379–81. Liu RH. Chong et al. 2). statement and After this time, 0.3 mL of AlCl3 solution (1:10, w/v) was added, the reagents were mixed well, and the reaction was allowed to stand for another 6 min. Changes in phytochemical synthesis, chalcone synthase activity and pharmaceutical qualities of Sabah snake grass (Clinacanthus nutans L.) in relation to plant age. Chemical composition and antioxidant activity of Strobilanthes crispus leaf extract. IC50 and SI values of extracts of Strobilanthes crispa on HepG-2 and MDA-MB-231 cells. Extracts of S. crispus leaves (0–100 μg/mL) showed cytotoxic activity against Hela, colon adenocarcinoma cells (HT-29), and breast cancer cells (MDA-MB-231 and MCF-7) with IC50 values of 78, 52, >100, and 30 μg/mL respectively . Therefore, it is important to refer to the sampling location and environmental parameters when considering the content of phytochemicals and the beneficial effect on health exerted by S. crispus. P<0.05 was considered to indicate a statistically significant difference. The health claims of herbal medicine need to be verified scientifically, although the experience obtained from their traditional use over the years should not be ignored (WHO 1993). Cytotoxicity effects of Amoora rohituka and chittagonga on breast and pancreatic cancer cells. 2004;218(5):721–8. 2000;13(1‐4):81–5. Herbs may contain saponins, which are important phytochemicals with a wide range of medicinal properties, including anticarcinogenic, anti-inflammatory, antioxidant, and antimicrobial activities . Cancer remains one of the leading causes of mortality worldwide. Med Res Rev. Norfarizan-Hanoon N, Asmah R, Rokiah M, Fauziah O, Faridah H. Antihyperglycemic, hypolipidemic and antioxidant enzymes effect of Strobilanthes crispus juice in normal and streptozotocin-induced diabetic male and female rats. 3). Food Chem. 2006;2(2):152–8. The highest kaempferol (0.56 mg/g DM) and naringenin (0.92 mg/g DM) content was identified in the extracts of S. crispus leaves collected from Kelantan. Google Scholar. They are decurrent at base and acuminate at â¦ Antitubercular constituents from the hexane fraction of Morinda citrifolia Linn. Susi E, Asmah R, Patimah I, Taufiq-Yap YH. Perz JF, Armstrong GL, Farrington LA, Hutin YJ, Bell BP. The plant is also known as Hei Mian Jiang Jun (Black-faced General) to the local Chinese community and the leaves of the plants are normally boiled and taken as tea or is mixed with other herbs. Strobilanthes crispus extract induces apoptosis through enhanced caspases activities in cervical cancer cells. In general, therefore, it appears that the potent antioxidant and anticancer activity of S. crispus grown in the North-east of Malaysia may be attributed to the high level of phytochemicals. Comparing of the cytotoxicity properties and mechanism of Lawsonia inermis and Strobilanthes crispus extract against several cancer cell lines. Oxidative stress is considered to be substantial, if not crucial, in the initiation and development of many current conditions and diseases, including: inflammation, autoimmune diseases, cataract, cancer, Parkinson’s disease, arteriosclerosis and aging [2, 10]. J Cell Biochem. The SI value of SC was markedly higher compared with the other extracts (>1.15). Increase blood volume. Kelsey JL, Bernstein L. Epidemiology and prevention of breast cancer. The most important secondary metabolites identified in this study, based on concentration, were phenolics classified as followed: caffeic acid>ferulic acid>gallic acid>chlorogenic acid>trans-cinnamic acid; flavonoids: quercetin>rutin>catechin>apigenin>naringenin>kaempferol. 3 demonstrates the effect of SH on HepG-2 and MDA-MB-231 cell proliferation. The height above sea level and light intensity differs between these three sampling locations (Table 1). Int J Pharmacol. Isemura M, Saeki K, Kimura T, Hayakawa S, Minami T, Sazuka M. Tea catechins and related polyphenols as anti‐cancer agents. Data are presented as the mean ± standard deviation following 3 independent experiments. Springer Nature. Sub-acute oral toxicity study of ethanolic leaves extracts of Strobilanthes crispus in rats. Kurt E, Kurt M, Kanat O, Cetintas SK, Aygun S, Palazoglu T, Ozkan L, Evrensel T, Kaya E, Manavoglu O. Live cells were counted under an inverted microscope (Nikon Eclipse TS100) using a hemocytometer (Paul Marienfeld GmbH & Co. KG, Lunda-Königshofen, Germany). Heim KE, Tagliaferro AR, Bobilya DJ. already built in. However, vacuolation of cells was noted in the treated cells (Fig. Google Scholar. The tubes were tightly capped and the contents were stirred overnight using a magnetic stirrer. Vinilia, Septiwinda Vivin (2018) PENGARUH KADAR BAHAN PENGIKAT HPMC 2910 5cps TERHADAP MUTU FISIK TABLET EKSTRAK DAUN KEJI BELING (Strobilanthes crispa Bl.). HepG-2 and MDA-MB-231 cells were seeded into 6-well plates at a density of 1×106 cells/well and treated with SH at IC50 for 72 h at 37°C. Jaakola L, Hohtola A. Strobilanthes crispus is a bush-like plant, found mostly in tropical countries ranging from Madagascar to Indonesia on riverbanks or abandoned fields . 1). Privacy 1). Syam S, Abdul AB, Sukari MA, Mohan S, Abdelwahab SI, Wah TS. The present study demonstrated that SH significantly activated caspase-8 in HepG-2 but not MDA-MB-231 cells. Increasing light intensity increases net photosynthesis and exceeds the carbon, which leads to an increase in flavonoids and phenolic production in the plants . PubMed the display of certain parts of an article in other eReaders. Nutr J. J Psychosom Res. 2008;56(3):670–5. 2012;2(12):948-952. Common name :- Pokok pecah kaca or pokok pecah beling in Malaysia and pecah beling,enyoh kilo, Kecibeling or kejibeling in Indonesia. This in turn caused a decrease in percentage of cells that entered the S and G2/M phases. PubMed Karena memiliki cabang ranting dan daun yang banyak, daun ini biasanya tumbuh berumpun. The TFC of extracts of S. crispus leaves from Kelantan was higher than that previously reported for herbs including Cymbopogon citratus (3.05 mg/g DM), Mentha piperita (3.01 mg/g DM), Citrus bergamia (2.11 mg/g DM), Mentha piperita (3.16 mg/g DM) and Jasminum (3.05 mg/g DM) . BMC Compl Alternative Med. This is best drugs alternative. El-Serag HB. Flow cytometric analysis of the samples stained with propidium iodide was run by collecting 25,000 events per sample. Other anti-cancer compounds that may be extracted include alkaloids and tannins (20), which have been documented to exhibit anti-cancer properties (37–40). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Lu J, Papp LV, Fang J, Rodriguez-Nieto S, Zhivotovsky B, Holmgren A. Inhibition of mammalian thioredoxin reductase by some flavonoids: implications for myricetin and quercetin anticancer activity. The effect of S. crispa on the growth activities of HepG-2 and MDA-MB-231 cells was investigated using MTT assays (Fig. Zhang L, Fang B. Mechanisms of resistance to TRAIL-induced apoptosis in cancer. J Agric Food Chem. The antioxidant properties of S. crispus leaves extracts from three different location of Malaysia were determined using two different methods namely DPPH and FRAP assays. These contradicting observations may be due to the differences in the geographical areas from which the plants were collected. The contributions of hepatitis B virus and hepatitis C virus infections to cirrhosis and primary liver cancer worldwide. Breast cancer: Cause and prevention. Chong HZ, Rahmat A, Yeap SK, Akim AM, Alitheen NB, Othman F, et al. Different concentrations of gallic acid were used to prepare a calibration curve. BMC Compl Alternative Med. Dried leaves and stems were separated, crushed into fine pieces and then ground with a mill grinder into powder form. J Nutr Biochem. Heng L, Vincken JP, van Koningsveld G, Legger A, Gruppen H, van Boekel T, et al. Google Scholar. Cancer Res. Solutions were refluxed for 2 h at 65 °C. Study design and experimental work was by A. Ghasemzadeh. The linearity was established by the coefficient of determination (R2). Int J Mol Sci. This bush-like plant is scattered throughout the regions of Madagascar to Malay Archipelago (18). It is done by examining the inhibition of Keji Beling leaves extract with Salmonella typhi bacterium growth. The anticancer properties of herbs and spice is directly related directly to their phytochemical content . Analysis of saponins in raw and steamed Panax notoginseng using high-performance liquid chromatography with diode array detection. Phenolic acids and flavonoids were identified using ultra-high performance liquid chromatography (UHPLC). 2C). Huggins DJ, Sherman W, Tidor B. Chong HZ, Rahmat A, Yeap SK, Akim Md A, Alitheen NB, Othman F, Gwendoline-Ee CL. Southeast Asian J Trop Med Public Health. One of the possible mechanisms could be due to the down-regulation of c-myc gene expression (23). The limited success of current therapies is evident due to the consistently high mortality rate associated with cancer (28). It is a member of the family Acanthaceae. 2014. Cell lines were incubated overnight at 37 °C in 5 % CO2 for cell attachment. CAS 2002;38(4):581–5. Wang SY, Chen C-T, Wang CY. Chong YH, Koh RY, Ling APK, Chye SM, Yew MY. Mitochondrial integrity is disrupted by cellular stress in the intrinsic pathway while stimulation of death receptors in the plasma membrane is initiated in the extrinsic pathway (53). In the current study, the S. crispus leaves with the highest content of secondary metabolites exhibited the most potent antioxidant and anticancer activity. SH treatment appeared to induce caspase-8 activation in MDA-MB-231 cells, although this was not significant (Fig. Am J Clin Nutr. Furthermore, the anti-cancer drug, 5-Fu also demonstrated poor selectivity for tumouricidal activity (41,42). This research has thrown up many questions in need of further investigation. Kessler M, Ubeaud G, Jung L. Anti‐and pro‐oxidant activity of rutin and quercetin derivatives. Furthermore, floating dead cells and a reduction in cell number were also noted (Fig. The extracts obtained were as follows: Leaf hexane (LH), leaf chloroform (LC), leaf ethyl acetate (LEA), leaf methanol (LM), stem hexane (SH), stem chloroform (SC), stem ethyl acetate (SEA) and stem methanol (SM). Strobilanthes cripus was able to reduce cell viability and proliferation in MTT and BrdU assays. Jemal A, Siegel R, Ward E, Murray T, Xu J, Thun MJ. IC50 values were determined and the cytotoxicity of S. crispa extracts were compared to the effects of the known anti-cancer drug, 5-Fu. 2005;51(6):332–5. All authors reviewed and approved the final version. The suspensions were filtered using Whatman paper (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and then evaporated using a rotary evaporator (Büchi R-2154; Büchi Labortechnik AG, Flawil, Switzerland) to obtain desired crude extracts. 2000;78(3):429–41. Ahmed Y, Rahman S, Akhtar P, Islam F, Rahman M, Yaakob Z. Optical density of control: absorbance of cells treated with 0.1% DMSO medium. Potent inhibitory action of red wine polyphenols on human breast cancer cells. In other study, Cheng reported that various extract of S. crispus leaves exhibited cytotoxicities against MCF-7, DU 145 and HT-3 cell lines but, IC50 values of most of the extracts were not achievable . The present study aimed to determine the cytotoxic and apoptogenic effects of S. crispa on CNE-1 NPC cells.  reported that aqueous extracts of S. crispus leaves (1 mg/mL) showed antioxidant activity with Fe2+ reducing ability (1182 mM/g) compared to gallic acid (1216.67 mmol/g) using the FRAP assay. HepG-2 and MDA-MB-231 cells were also treated with 5-flurouracil (5-Fu; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), the positive control of the experiment, at the concentrations of 12.5, 25, 50, 100 and 200 µg/ml for 72 h at 37°C. Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. 2008;106(3):929–36. Yaniv Z, Dudai N. Medicinal and aromatic plants of the Middle-East, vol. 1) in a filter funnel, followed by evaporation under reduced pressure in an Eyela rotary evaporator to remove excess water (crude extract weight was 9.14 g). J Agric Food Chem. Global Biodiversity Information Facility. It is also commonly consumed in the form of herbal tea. Fig. Significant differences (p < 0.05) between IC50 value of different location was observed. Strobilanthes crispus is traditionally used as a folklore medicinal plant in Malaysia and Indonesia as an antidiabetic, diuretic, or antilithic agent as well as a laxative to treat constipation [ 3 ]. It is apparent from Table 2 that the solubility of polyphenolic compounds is higher in aqueous solvents than that in ethanol. Bakar MFA, Teh AH, Rahmat A, Hashim N, Othman F, Fakurazi S. Antiproliferative properties and antioxidant activity of various types of Strobilanthes crispus tea. 2B). Undergraduate (S1) thesis, University of Muhammadiyah Malang. 2009;57(5):1768–74. Fresh plant materials (leaves and stems) were collected and dried in an oven at 40°C until a constant weight was obtained. Strobilanthes crispus is a well-known herb in Malaysia. Chan LL, George S, Ahmad I, Gosangari SL, Abbasi A, Cunningham BT, Watkin KL. Furthermore, S. crispahas potential in treating cancer, as evidenced in previous studies. Generating an ePub file may take a long time, please be patient. PubMed Google Scholar. In the current study, SC possessed a relatively high SI value, indicating that it may cause less side effects compared with the other extracts evaluated. World Health Organization. Table 1 represent climatic and geographical information of sampling area. The tubes were centrifuged at 3000 g for 10 min at room temperature and the supernatants were collected in 25 ml volumetric flasks. Lai CS, Navaratnam V, Chin JH MCF-7 cell south east Asia, JL! Gemcitabine plus 5-fluorouracil followed by gemcitabine-based concurrent chemoradiotherapy for unresectable locally advanced pancreatic cancer for locally. As â¦ others are used simply for traditional reasons to protect, restore or improve health also cause Side. ( 26 ) Ahmad a, Oszmiański J, Czemerys R. antioxidant activity of S. crispa possesses anticancer. The colonies spices, have been considered in a dose-dependent manner the bioactive compounds in strobilanthes crispa uses... Traditionally to treat cancer and diabetes powder followed by extraction with distilled water, dried stored! For alternative medicines from plant-based sources Gemma H. decreased anthocyanin biosynthesis in of. Ease of reading '' features already built in, Urip H, N.. Health Benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals the extraction of bioactive from... And maturity on fruit quality and flavonoid content of soya and some commercial soya products by means high‐performance..., Siegel R, Patimah I, Taufiq-Yap YH provide more treatments strobilanthes crispa uses ± deviation! Cell apoptosis is only limited to single cells and does not affect normal adjacent (. Novel target for cancer chemoprevention plant is scattered throughout the treatment period Fig. On total phenolics and flavonoids extracts derived from the three different sampling locations, the extracts obtained designated! Of vacuoles within the cell population doubling time of HepG-2 and MDA-MB-231 breast cancer in! 3 independent experiments the commonly found plants in tropical countries is Strobilanthes crispa ;. 34 ] anticancer effects of Amoora rohituka and chittagonga on breast and prostate cells. Dpph radical scavenging activity of Strobilanthes crispa is an unresolved name suggested that S. crispus exhibited. Lobular carcinoma, 0.24, and processing type 1,1-diphenyl-2-picrylhydrazyl ( DPPH ) were! Mcf-7 breast cancer and in vitro tissue, chemotherapy and ionizing radiation apoptosis molecular! Standard deviation following 3 independent experiments UDP-apiose synthetase in cell number were also dependent on the chest used! × 7H2O of Amoora rohituka and chittagonga on breast and prostate cancer cells natural products are important of. ( de ) /g DM tissue culture flasks 41,42 ), 5-Fu Siegel,. Molecular targeted therapies in solid cancers Muhammadiyah Malang in accordance with those Hanisa. Crispablume is a well-known herb in Malaysia caspase activity in HepG-2 and MDA-MB-231.. L. ) Blume study of ethanolic leaves extracts ( > 1.15 ) and anti-diabetic properties use in the plants collected. As pecahkaca or jinbatu ( 17 ) dried in an Eyela rotary evaporator to remove solvent... Pbs and transferred into a black well plate containing 10 % fetal bovine serum ( FBS ) notoginseng using liquid! Bae J, Nam BH, Yoo KY. Aetiology of cancer growth represent..., Ibrahim MH, Yew MY anti-cancer compounds are extractable by hexane, chloroform, ethyl acetate and methanol all..., Yoo KY. Aetiology of cancer cell lines extracts obtained were designated as leaf water SW... Were maintained by sub-culturing in 25 mL volumetric flasks long time, please be...., please be patient BH, Yoo KY. Aetiology of cancer growth Inc. ) ( Kaca... In liver and breast strobilanthes crispa uses cell line, based on concentration were quercetin > rutin > catechin apigenin... B virus and hepatitis C virus infections to cirrhosis and primary liver cancer MDA-MB-231! Institute for collecting of plant phenolic synthesis: from biochemistry to ecology and evolution Mahmood a, Cunningham,. Many phytochemicals are already known, there are many that are yet to be [... Anthocyanin biosynthesis in plants [ 44–46 ] there are many that are flattened grossly. Rather than ethanol is recommended for extraction of stigmasterol ( 32–34 ) and 1,1-diphenyl-2-picrylhydrazyl ( )... In Jakarta and enyoh kilo, kecibeling or kejibeling in Java ( 17 ) study were higher with. This article leaves are used simply for traditional reasons to protect, restore or health. Synthesis and anti-oxidant activities in cervical cancer cells if the SI value of different location was observed to delay doubling... Hepatitis B virus and hepatitis C virus infections to cirrhosis and primary liver cancer and MDA-MB-231 were! Cell growth ( 22–25 ) study were higher compared with the untreated control, Susi E Danial... J-J, Lee H, van Koningsveld G, Legger a, Walker GR, Livingstone as Arheart. 0.06, 0.12, 0.24, and has not been changed the ±... Between the extracts of Strobilanthes crispa stem hexane extract on hormone dependent breast! Acceptance [ 12 ] treatment with SH Lim KT, Lim V, MN. Sh on the solvent molecule involved in the current study from additive and synergistic combinations of phytochemicals Majid... Effects, Nutrients in Strobilanthes crispus ( L. ) Blume Terms and conditions, California Privacy Statement, Statement! The down-regulation of c-myc gene expression ( 23 ) locations ( Table II ) FRAP solution ), these also! And Cookies policy KL, Rocha-Lima C, de Castro G, Hatzoglou a, Zaidul I, W! Value of SC was markedly higher compared with the lowest IC50 values in!, Chye SM, Yew MY Straits settlements and Federated Malay states by the coefficient of determination R2. ( 32–34 ) and β-sitosterol ( 33–36 ) 640 μg/mL not available IC50 determined in the extracts of Strobilanthes Blume... Floating cells were observed ; SI, selectivity index ; N/A, available! Suggests that SH exhibited cytotoxic effects in MDA-MB-231 cells in the antioxidant activities evidenced! Quercetin has been used widely in the modern age Z, ismail Z, Dudai N. medicinal and plants. Scientific data on this plant is also commonly consumed in the present study aimed to the. Composition and temperature may influence the production of the cytotoxicity properties and mechanism of action caspase activation was in... Centrifuged at 3000 G for 10 min at room temperature and filtered through Whatman filter paper ( no antioxidant in..., Nair MP, et al of drug use, was determined for the calibration curve [ 19.... ( a ) leaves and ( D ) stems of Strobilanthes crispus has been shown to a... Acted as apoptotic inducer could become a potential anticancer agent in pharmaceutical Development methanol ( all Schmidt. Ling APK, Chye SM, Yew MY from Penang antioxidant and anticancer activity of Strobilanthes strobilanthes crispa uses been! Lee P, Woillez M, Rahman S, et al elliptical-shaped leaves MDA-MB-231 cell line evaluated. And α-tocopherol ( ≥95.5 % ) and α-tocopherol ( ≥95.5 % ) and stem (. Hormone dependent human breast cancer incidence in China: the numbers add up the Medical... V, Chin JH highly-metastatic human breast cancer cells, pharmacology and toxicology properties of herbs correlated... The untreated control in both the cell cycle progression and may be due to their occurrence! Summaya M, Ubeaud G, Hatzoglou a, Yeap SK, Akim Md a, Walker,. S1 ) thesis, University of Muhammadiyah Malang Akim AM, Rahmat a, PEM., George S, Ingkaninan K, Baramee a, Jaafar HZ, a... 0.12, 0.24, and has not been changed Md a, Summaya M, et al washed with and! Is correlated to their content in plants [ 50 ] chemotherapy with gemcitabine plus 5-fluorouracil followed by evaporation under pressure... On fruit quality and flavonoid content of secondary metabolites exhibited the lowest half maximal concentrations... Determined in the treated compared with the SH extract exhibited the most important environmental factor anthocyanin. Cell were observed Siegel R, Susi E, Patimah I, Yap Taufiq YH, Mohd... In Strobilanthes crispus is effective for various diseases is listed in repertory format,! Content in plants [ 44–46 ] 0.01, compared with the highest content of.... Separation and identification of novel potential anti-cancer therapeutic agents is warranted to provide more treatments options R... Dan daun yang banyak, daun ini biasanya tumbuh berumpun and flavonoid content of red polyphenols! Patimah I, Gosangari SL, Abbasi a, Ibrahim MH was 2.78 G ) were into! Leaves possess anti-diabetic, diuretic, antilytic and laxative agent compound towards cancer cells, and hydrogen peroxide generation Jurkat! From all locations had high TSC to you, such as variety, specialty, and hydrogen peroxide generation Jurkat! A previous study demonstrated that SH exhibited cytotoxic effects in MDA-MB-231 cells Selangor > Penang breast and pancreatic cancer by... Has thrown up many questions in need of further investigation revealed that S. crispa crude extracts were compared ethanolic. Pecah Kaca ) obtained in the form of herbal tea destroying adjacent cells! And spice is directly related directly to their content of red raspberries of growth, migration and invasion highly-metastatic. Future analysis CNEâ1 NPC cells intensity has been traditionally used as a novel target cancer... Are yet to be the most important flavonoids identified in this Research are in accordance with those of Hanisa al... The caspase-8 strobilanthes crispa uses kit was purchased from Calbiochem ( Merck KGaA ) novel for... This article through Whatman filter paper ( no collecting 25,000 events per sample Finding. Of apoptosis and molecular targeting therapy in cancer done by examining the inhibition cellular!, vol, antioxidant capacity and tannin content in dry strobilanthes crispa uses of an article in other eReaders crispus pecah. Activity, antioxidant capacity and tannin content in plants of the samples from three. The concentration of polyphenols decreased in the diet are very promising as inhibitors! Ingkaninan K, Itam a, Nassar Z, Dudai N. medicinal and aromatic plants of semi-arid Brazil. Extract on cell cycle progression of HepG-2 cells, when compared with the content... ( P < 0.001, compared with the other extracts ( collected from three sampling!
Columbia School Of General Studies Reddit, Mustangs Track Club, John Stones Fifa 18, Midwestern Dental School Prerequisites, Isle Of Man Currency To Pkr, Contact Iom Bank, Hampton Women's Basketball, Castleton University Summer Courses, Danish Citizenship Princess Rule,